實驗室簡介： 分子酶學(xué)與合成生物學(xué)實驗室依托于上海交通大學(xué)微生物代謝國家重點實驗室，致力于解析酶催化與分子進化規(guī)律，發(fā)展酶定向進化技術(shù)體系，并開發(fā)酶在工業(yè)、醫(yī)藥等領(lǐng)域的重大創(chuàng)新應(yīng)用。 實驗室承擔(dān)了國家973計劃項目、863項目、國家重大研究計劃項目、自然科學(xué)基金委重大儀器專項、自然科學(xué)基金等研究任務(wù)。在Science Adv., Nature Commun., Angew. Chem., JACS, Anal Chem, J. Biol Chem, 等著名雜志發(fā)表多篇論文。 代表性論文： 1)Xi Deng, Liuqing Chen, Mohan Hei, Tiangang Liu, Yan Feng, Guang-Yu Yang. Structure-guided Reshaping of the Acyl Binding Pocket of 'TesA Thioesterase Enhances Octanoic Acid Production in E. Coli, Metab Eng. 2020, 61:24-32. 2)Yumeng Tan, Yong Zhang, Yunbin Han, Hao Liu, Haifeng Chen, Fuqiang Ma, Stephen G Withers, Yan Feng, Guangyu Yang. Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method. Science Advances, 2019, eaaw8451 3)Fuqiang Ma#, Meng Ting Chung#, Yuan Yao, Robert Nidet, Lap Man Lee, Allen P. Liu, Yan Feng, Katsuo Kurabayashi*, Guang-Yu Yang*. Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform. Nature Communications, 2018, 9:1030. (#equal contribution) 4)Hoque MA, Zhang Y, Chen L, Yang G, Khatun MA, Chen H, Hao L, Feng Y*. Stepwise Loop Insertion Strategy for Active Site Remodeling to Generate Novel Enzyme Functions. ACS Chemical Biology. 2017, 12(5):1188-1193. 5)Zhuang Y#, Yang GY#, Chen X, Liu Q, Zhang X, Deng Z, Feng Y*. Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme. Metabolic Engineering. 2017, 42:25-32. (#equal contribution) 6)Han YB, Chen LQ, Li Z, Tan YM, Feng Y, Yang GY*. Structural insights into the broad substrate specificity of a novel Endo-glycoceramidase I belonging to a new subfamily of GH5 glycosidase. J Biol Chem. 2017, 292(12):4789-4800. 研究課題 1. 利用結(jié)構(gòu)生物學(xué)、家族進化樹分析、祖先基因重構(gòu)等方法揭示自然界關(guān)鍵酶家族的進化規(guī)律，探討酶高效的催化功能與底物選擇性的形成機制與調(diào)控規(guī)律； 2. 開發(fā)蛋白質(zhì)從頭設(shè)計、微流控酶活性超高通量篩選等酶分子設(shè)計與定向進化的新技術(shù)方法，提升工程化酶人工構(gòu)建與優(yōu)化的效率； 3. 針對具有重大實際應(yīng)用需求對關(guān)鍵酶進行酶分子設(shè)計與定向進化，提高酶對非天然底物的選擇性、催化效率及其對環(huán)境的適應(yīng)性，促進酶在生物醫(yī)藥領(lǐng)域的應(yīng)用。

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